| Component |
Techniques |
Investigator |
| Project management |
|
Hickey, Trainer |
| Currents |
Moored arrays, ADCP surveys |
Hickey, Thomson |
| Winds |
Moored arrays, NDBC/EC buoys, MM5 model |
Hickey, Foreman |
| Surface currents |
Drifters |
Hickey |
| Standard water properties (T, S, Fluor., PAR, O2), PN abundance |
Surveys, flow-through fluorometry, T, S, FlowCAM, CTD, moored
arrays |
Hickey, Cochlan, Lessard |
| Chl a and Phaeopigments |
In vivo and in vitro fluorometry |
Trainer, Cochlan |
| Domoic acid (particulate and soluble) |
Receptor binding assay, Mist Alert, cell bioassay |
Trainer, Trick |
| Dissolved nutrients (inorganic and organic) |
Lechat autoanalyzer, surveys and moored samplers |
Cochlan, Trick |
| Fe, other trace metals |
Flow injection, extraction/ICP-MS |
Wells |
| Pico and nanoplankton |
FCM |
Trick |
| Autotrophic/heterotrophic microplankton |
FlowCAM, microscopy/image analysis |
Lessard |
| Pseudo-nitzschia taxonomy |
SEM, probes |
Trainer |
| Molecular probe development |
PCR |
Connell |
| Antibody and assay development |
Polyclonal, cell fluorescence |
Trainer, Trick |
| Toxic Pseudo-nitzschia species abundance, rRNA probes |
Sandwich hybridization assays (SHA); FISH, FCM |
Trainer, Connell, Lessard, Trick |
| Nitrogen uptake capabilities |
15N nitrogen kinetics (NO3, NH4, urea) |
Cochlan |
| Nutrient stress and DA production |
Modify nutrient ratios incubation expts. |
Trick, Cochlan, Trainer |
| Fe, Cu stress and effects on DA production as a chelator |
Competing ligand 'grow-outs', Fe uptake kinetics, P vs. E expts.
using 14C |
Cochlan, Wells, Trick, Trainer |
| Grazing/growth of Pseudo-nitzschia and other phytoplankton |
Dilution method - FlowCAM, microscopy, SHA, size-fractionated
chl a |
Lessard |
| Identity of Grazers of Pseudo-nitzschia species |
Single-cell FISH and immunoassays |
Lessard |
| Bacterial abundance and productivity |
AO counts; 3H-leucine uptake |
Cochlan |
| Circulation modeling |
Numerical model |
Foreman |
| Trophic modeling |
NPZ model |
Peña |
| Biophysical modeling |
1-D and 3-D models |
Foreman, Peña |
